Coding

Part:BBa_K3282000:Design

Designed by: Manjyot Kaur, Mateusz Piotr Szewczyk   Group: iGEM19_Lund   (2019-10-11)


ArsR from E. coli K12 codon-optimized for E. coli Nissle 1917


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was codon optimized for E. coli Nissle 1917


Source

NCBI Reference Sequence: NC_000913.3 (3648528..3648881)

References

1. Carlin, A., Shi, W., Dey, S., Rosen, B. (1995). The ars operon of Escherichia coli confers arsenical and antimonial resistance. Journal of Bacteriology, 177 (4), 981-986; doi: 10.1128/jb.177.4.981-986.

2. Rastorguev, S.M., Zavilgelsky, G.B., Tchurikov, N.A. (1998). IncI1 plasmid R64 encodes the ArsR protein that alleviates type I restriction. FEBS Lett. 426, 21–23.